Molecular Weight: 610.59. Shop STAT5 STAT5 alpha Mouse anti-Human, Mouse, Rat, Clone: ST5a-2H2, Invitrogen 100 g; Unconjugated Primary Antibodies Ss to St | Fisher Scientific English After the cells completely adhered to the well, oleic acid (0.6 mmoL/L) [] was added, and the cells were incubated at 37 C for 72 h. STAT5. This antibody is specific to PFKFB3. Download figure Download PowerPoint Figure 2. quantity: 100 g price: 543 USD Purity is >95% by SDS-PAGE. This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Sarah is an Entrepreneur in Residence for the Texas Medical Center Accelerator for Cancer Therapeutics (TMC ACT). STAT5-IN-1, CAS 285986-31-4 Please contact us for prices and availability for the specification of product you are interested at. Western Blotting and Molecular Reagents. STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. Molecular Weight. Each pooled sample in D was derived from equal amounts of the four . What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol? 1 the chemokine domain contains 2 cysteines separated by 3 other amino acids (cxxxc) and is therefore designated Signal transducers and activators of transcription (STATs) play key roles in growth factor-mediated intracellular signal transduction. . Molecular Weight: 610.59. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes . The main MDM2 transcript in human cells codes 491 amino acids with a 90-kDa molecular weight protein and a RING finger motif on its C terminus, responsible for the E3 ubiquitin ligase activity . Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). Clone 89/Stat5. For example, STAT5 and ERK1/2 phosphorylation can be measured using antibodies specific for the phosphorylated version of these molecules in combination with flow cytometry analysis as described herein . Irrespective of the ability of NK cells to recognize and eradicate tumor cells . In the present study using a constitutively active STAT5 mutant, we show that STAT5 has pleiotropic functions regulating cell proliferation, differentiation and apoptosis in an IL-3-dependent Ba/F3 cell line. Description SDS Pricing; 5.09105: A cell-permeable BP-1-102 analog that targets STAT3 and STAT5 with comparable affinity. Leukemia stem cells (LSCs) constitute the critical barrier to the cure of acute myeloid leukemia (AML) due to their chemoresistance and immune evasion property. Then virtual screening based on molecular docking and molecular dynamics was applied to screen a set of ~1500 compounds for dual inhibitors of the SH2 domains of STAT5 and STAT3. This cytokine induces the proliferation of natural killer cells . Contents Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO. Target. Arrow points to STAT5 (molecular weight 90 kDa). Though STAT5A and STAT5B are encoded by separate genes, the proteins are 90% identical at the amino acid level. accumulation. Extended Data Fig. Target Molecular Weight: 92 kDa Concentration: 250 g/ml RRID: AB_399386 Storage Buffer: . STAT5-IN-1 inhibits STAT5 by binding to the SH2 domain. Positive Controls: BJAB whole cell lysate: sc-2207, CCRF-CEM cell lysate: sc-2225 or K-562 whole cell lysate: sc-2203. MT-2 cells were treated with NC1153 as indicated for 10 h. ChIP assays were performed with anti-STAT5 antibody and IgG control from each treatment. Whereas IL-5 activated the wild-type STAT5A and STAT5B proteins in HL60-eos cells, a carboxyl-terminally truncated 80-kDa STAT5 isoform was activated in mature eosinophils and neutrophils. Cell Signaling Stat5 (D2O6Y) Rabbit mAb 20 ul - CSIG (Additional S&H or Hazmat Fees May Apply) Cell Signaling Stat5 (D2O6Y) Rabbit mAb ; CSIG-94205T The store will not work correctly in the case when cookies are disabled. Cell Culture and Modeling. . After dosing in mice, the larger molecular weight of ALKS 4230 results in decreased clearance and translates to a longer half-life compared with rhIL-2, enabling less frequent dosing. View More View Less IC 50 & Target. Activation of STAT5 A/B in MCF-7 cells Whole cell lysates of untreated (lane 1) and pervanadate-treated (lane 2) MCF-7 cells were applied to SDS-PAGE and transferred to a PVDF membrane. Enter 197.13 into the Molecular Weight (MW) box This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Although Stat5 has pleiotropic physiologic functions [Nosaka T et al. Learn more about SELLECK CHEMICALS LLC MS. We enable science by offering product choice, services, process excellence and our people make it happen. Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Target Molecular Weight: 92 kDa Concentration: 250 g/ml RRID: AB_397590 Storage Buffer: . In these studies, we examined signaling through the transcription factor STAT5 in human peripheral blood eosinophils after treatment with granulocyte macrophage colony-stimulating factor . Storage & Handling Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b, which share over 90% identity at the amino acid level. J Biol Chem. 95%. . Herein, the role o Not for Human or Veterinary or Therapeutic Use. Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Protocols. Prematurely born infants often require supplemental oxygen that impairs lung growth and results in arrest of alveolarization and bronchopulmonary dysplasia (BPD). Target Information STAT5 alpha is a member of the STAT family of transcription factors. Molecular weight is represented by the same number in all unit systems . We collected "control pts" with similar characteristic (n=20). Rabbit recombinant monoclonal STAT5 (phospho Y694) antibody [E208]. b , Immunoblotting of Flag-IL2R in 293 T cells transfected with . (B-E) STAT5-IN-1 failed to alter HFD-induced body weight changes (B) and serum lipid levels (C-F). Validated in WB, ELISA, IHC and tested in Human. Cited in 50 publication(s). The immunoblot was probed with mab STAT5-5G4 at 0.5 ug/ml for 1h at 15-22C and developed by ECL (exposure time: 5 sec). BD750 is an immunosuppressant and a dual inhibitor of JAK3 and STAT5 that inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation with IC50 of 1.5 M and 1.1 M for mouse and human T-cell proliferation, respectively. Arrow points to STAT5 (molecular weight 90 kDa) Filter by Application: WB Mouse anti STAT5 Product Type PrecisionAb Monoclonal Clone STAT5 knockout during the first 10 days of lactation decreases neonatal body weight by 30-40% due to changes in mammary gland morphology and a reduction in milk production. We show that different STAT5 isoforms are activated by IL-5 and GM-CSF in eosinophils, neutrophils, and differentiated eosinophilic HL-60 cells. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. All products from TargetMol are for Research Use Only. Western blot analysis of whole cell lysates probed with STAT5 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 9 second exposure. Klionsky DJ. (Help) Allow more atoms of elements in formula than specified. Gene Wiki entry for STAT3 Gene Additional gene information for STAT3 Gene STAT5-IN-1 is a STAT5 inhibitor with an IC50 of 47 M for STAT5 isoform. The STAT5 induction by IL-3 is shown in Figure 1. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC . 1/1000. Pathway. Anaplastic Large Cell Lymphoma (ALCL), characterized by expression of CD30, refers to a family of Non-Hodgkin T cell lymphomas divided into four entities: systemic Anaplastic Lymphoma Kinase (ALK) positive (ALK + ALCL), systemic ALK negative (ALK ALCL), primary cutaneous ALCL (pc-ALCL) and breast-implant associated (BIA-ALCL) [ 1 ]. STAT5 Inhibitor is a cell-permeable, nonpetidic nicotinoyl hydrazone that suppresses STAT5 by targeting its SH2 domain (IC50 = 47 M against STAT5?? Compare Product No. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). The mutant STAT5 induces IL-3-independent DNA binding activity without activation of JAK2. Samples were incubated with Phospho-STAT5 alpha (Ser780) polyclonal antibody ( Product # PA5-36891 ). (A) Western blotting showed that p-STAT5 level in artery tissues of ApoE -/- mice induced by HFD was decreased by STAT5-IN-1. Novel mechanism for FcRI-mediated signal transducer and activator of transcription 5 (Stat5) tyrosine phosphorylation Description: This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. Molecular Weight of Stat5: 92 kDa. Product Details Product Specific Information This antibody detects endogenous protein at a molecular weight of 90 kDa. We previously showed that body fat in both groups falls to <2% of body weight by day 5 on this diet . STAT5 and ERK/2 signaling can be measured, for example, by phosphorylation of STAT5 and ERK1/2 using any suitable method known in the art. Formerly, she was a cofounder and the Vice President of Operations at Courier . Please follow the steps below to conduct your search (Help) : Enter a molecular weight value or range: If desired, enter a formula to restrict the search: Allow elements not specified in formula. After 10 days, the tumor weight of Stat5 fl/fl, . Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain and the common gamma chain (gamma-C, CD132).IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). Log in . Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). Streuli CH, Edwards GM, Delcommenne M, Whitelaw CBA, Burdon TG, Schindler C, Watson CJ. Nat Rev Mol Cell Biol. Expand. AC-4-130 directly binds to STAT5 and disrupts STAT5 activation, dimerization, nuclear translocation, and STAT5-dependent gene transcription. NOTE: Loading of prestained molecular weight markers (#59329, 10 l/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 l/lane) . *The above data is based on the productmolecular weight 293.3. Pullen, N.A., et al. (n=12 in each group; ns, no significance, vs. HFD). Function. Use a concentration of 1 g/ml. The 1 * 6 mutant STAT5 has two mutations; one in the activation domain (designated mutation 1) and the other in the DNA binding domain (mutation 6). Expand. for p-STAT5 and t-STAT, 60 g was loaded. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and liver gene expression. Therefore, we investigated ISO's effect and explored its potential underlying molecular mechanisms in AD. There are two distinct Stat5 genes, Stat5a and Stat5b. Expand. Description SDS Pricing; 5.09105: A cell-permeable BP-1-102 analog that targets STAT3 and STAT5 with comparable affinity. Stat5 as a target for regulation by extracellular matrix. Stat5 mRNA quantification was significantly diminished in pt samples at M6 and M12 compared to the baseline values and a reduction of the clonogenic potential was also observed in bone marrow cells at M6 and M12. 13, 14 Home Tools. 5 The authenticity of the IL-2R molecular weight. b STAT5-mediated expression of c - Myc in Ba/F3-WT and -1*6 cells. In the SI system the unit of M is [kg/kmol] and in the English system the unit is [lb/lbmol], while in the cgs system the unit of M is [g/mol]. contribute to malignant transformation [de Groot RP et al. The positions of the 72 kDa molecular weight proenzyme and 62 kDa molecular weight active enzyme are indicated on the left side of the panels. Enter the email address you signed up with and we'll email you a reset link. Expand. The STAT5 Inhibitor, also referenced under CAS 285986-31-4, controls the biological activity of STAT5. 794 amino acids, predicted molecular weight of 91 kD; contains . taken together, these data (figures 5 - 7) identified two aspects of stat5 biology relevant in the pathogenesis of ph: first, the contribution of differential male versus female py-stat5 activation patterns in the early stages of this disease to generating the sex bias, and second, the loss of stat5 in cells in vascular lesions in many patients AC-4-130 induces cell cycle arrest and apoptosis in FLT3-ITD-driven leukemic cells. STAT5 DNA binding is expressed as fold change over background (IgG control). The growth hormone (GH)- and insulin-like growth factor (IGF)1 systems regulate cell homeostasis and organ development. Stat5-IN-2 | C26H27N3O | CID 137628635 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities . STATs are activated by phosphorylation by JAKs. [1] Despite the potential for STAT5 phosphorylation in T regs, . AC-4-130 is a potent STAT5 SH2 domain inhibitor. . View all Formats . STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant. Results were expressed as the mean SEM, original magnification, 400, bar = 100 m. They were cultured in DMEM at 37 C with 5% CO 2.The cells were digested and harvested, then diluted and inoculated in a 24-well plate (3 10 4 /well). Interaction Forms a homodimer or a heterodimer with other family members. . The molecular weight of a substance, also called the molar mass, M, is the mass of 1 mole of that substance, given in M gram. In contrast, . Product Specific Information. (B) JAK3 blockade diminishes BCL10-SBR occupancy by STAT5 in vivo. IP-MS Validated Western blot analysis of whole cell lysates probed with Mouse anti Human STAT5A antibody, clone OTI6D6 ( VMA00712) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 15 second exposure. fractalkine (fkn) is a unique membrane-bound molecule expressed on endothelial cells (ecs), possessing a chemokine domain and an extended mucin-like stalk that allows it to function as both a chemoattractant and an adhesion molecule. STAT5 Inhibitor is a cell-permeable inhibitor which suppresses Stat5 via binding to the SH2 domain. . Hide. 1995; 270:21639-21644. doi: 10.1074 . Hide. STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin. The results were quantitated as pSTAT5 positive colonic IECs per Crypt. Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. STAT5A/B signal in parental Ba/F3 cells corresponds to endogenous STAT5 protein levels, while the signals detected in the stable cell lines Ba/F3-WT and Ba/F3-1*6 represent both endogenous (STAT5A and STAT5B) and transgenic (STAT5A-WT or -1*6) proteins. 47 M (IC 50) In Vitro. Articles of STAT5-IN-1 are included as well. Anti-STAT5a antibody [E289] (ab32043) Research with confidence - consistent and reproducible results with every batch Long-term and scalable supply - powered by recombinant technology for fast production Success from the first experiment - confirmed specificity through extensive validation Ethical standards compliant - production is animal-free Their activation is often an integral component of redundant cytokine signal cascades involving complex cross-talk and pleiotropic gene regulation by Stat5 has been implicated in cellular functions of proliferation, differentiation and apoptosis with relevance to processes of hematopoiesis and immunoregulation, reproduction, and lipid metabolism. The store will not work correctly in the case when cookies are disabled. The molecular weights reported elsewhere in this site are based on average atomic masses. 2.1. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). STAT5-IN-2 is an inhibitor of STAT5 (EC50s: 9 M and 5 M in K562 and KU812 cells, respectively). Molecular weight markers are indicated to the left. DATA SELECT PRODUCT CITATIONS 1. From: Advances in Immunology, 2016 Download as PDF About this page Erythropoietin Robert T. Mallet, Myoung-Gwi Ryou, in Vitamins and Hormones, 2017 Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation. Chemsrc provides STAT5-IN-1(CAS#:285986-31-4) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Description. Compare Products ; Menu . Stat5 is constitutively active in some leukemic cell types (4). Purified Mouse Anti-Stat5. Target. Calculated molecular weight: 520 aa, 60 kDa: Observed molecular weight: 58 kDa: GenBank accession number: BC040482: Gene symbol: PFKFB3: Gene ID (NCBI) . . Compare Product No. Since IGF1 is decreased in preterm infants, we investigated the GH- and IGF1 signaling (1) in newborn mice with . 1999]. ( A) Structure of the constitutively active mutant STAT5. Anti-phosphotyrosine antibodies detected a protein of molecular weight 92 to 97 kD from cytokine-stimulated eosinophils. Interleukin-15 (IL-15) is a cytokine with structural similarity to Interleukin-2 (IL-2). The signal transducer and activator of transcription 5 (STAT5) is a member of the STAT family of proteins, implicated in cell growth and differentiation.

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